Estrogen Receptor (ER) Transcriptional Activation Analysis through SEP (Surface Plasmon Resonance) and DEST (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay)
The Estrogen Receptor Transcriptional Activation (Human Cell Line HeLa-9903) Assay is a significant tool in evaluating chemical activity in relation to the estrogen receptor. This assay, originally developed by the U.S. Environmental Protection Agency (EPA), employs the human HeLa-9903 cell line and two key components: the Standard Evaluation Procedure (SEP) and the Data Entry Spreadsheet Table (DEST).
The Standard Evaluation Procedure (SEP) is a comprehensive protocol document that outlines the process for evaluating and interpreting data generated from the assay. It establishes criteria for data quality control, activity calling, curve fitting, handling of replicates and controls, filtering, and interpreting results for chemical hazard or risk assessment purposes. The 2011 version of the SEP integrates improvements in data handling and interpretation to ensure consistency and reliability.
On the other hand, the Data Entry Spreadsheet Table (DEST) is an Excel-based spreadsheet or database template designed to capture raw and processed data from the assay. It facilitates standardized data entry, storage, and subsequent analysis, including fields for chemical identification, experimental conditions, raw readings, curve fit parameters, quality control metrics, activity calls, notes, and comments. Using the DEST helps ensure data consistency and facilitates integration into larger databases such as EPA’s ToxCast program.
Both the SEP (September 2011) and the DEST (September 2011) are available as digital documents. The SEP is available as a pdf file, while the DEST is available as an xlsx file. The file size of the SEP is 176.84 KB, and the DEST is 39.76 KB.
For those requiring the actual documents or templates, these were originally published and managed by the EPA’s Endocrine Disruptor Screening Program (EDSP) and often integrated within the ToxCast/Tox21 datasets. To obtain the official files, you may need to consult the EPA or related scientific repositories directly.
The Estrogen Receptor Transcriptional Activation (Human Cell Line HeLa-9903) Assay aids in evaluating potential health-and-wellness implications, particularly medical-conditions like cancer, due to its assessment of chemical activity related to the estrogen receptor. The Standard Evaluation Procedure (SEP), a crucial component of the assay, provides a comprehensive approach for data interpretation and establishes quality control criteria for hazard or risk assessment purposes, as highlighted in the 2011 version of the SEP.
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