L-arginine synthesized through fermentation by Corynebacterium glutamicum strain KCCM 80393
In a recent application, the determination of L-arginine in feed additives, premixtures, compound feed, and water has been proposed to be carried out using a ring-trial validated European Union (EU) method. This method, based on ion exchange chromatography (IEC) coupled with post-column derivatisation and optical (VIS) detection, was initially validated for several amino acids, including threonine, cyst(e)ine, methionine, lysine, and later expanded to include arginine.
The European Union Reference Laboratory (EURL) and the Community Reference Laboratory (CRL-FEED) have both endorsed this method for official control purposes. It is a high-performance liquid chromatography (HPLC) technique that typically involves cation exchange chromatography with post-column ninhydrin derivatization to detect amino acids like L-arginine.
The method is designed to provide accurate, precise, and reliable quantification, consistent with EU regulatory requirements for feed safety and labeling. The EU method is applicable for the determination of L-arginine in various feed-related matrices, including feed additives, premixtures, compound feed, and water.
The authorization for L-arginine, produced by fermentation with Corynebacterium glutamicum KCCM 80393, is sought under Article 4(1) for all animal species. The feed additive contains a minimum L-arginine content of 98.5% (w/w, based on dry matter).
The EURL also considers the ring-trial validated EN ISO 17180 method based on IEC-VIS/FLD as applicable for the determination of arginine in the feed additive and premixtures. This method complements the EU method in ensuring consistent control and verification across member states, as it is referenced in official EU documents and described as the standardized approach for amino acid analysis in feed.
The validation studies for the EU method demonstrate a relatively low RSD for repeatability and reproducibility, indicating its accuracy and precision for the determination of L-arginine. For identification purposes, the EURL recommends the "L-arginine monograph" of the Food Chemical Codex (FCC).
In conclusion, the proposed EU method offers a reliable and standardized approach for the determination of L-arginine in various feed-related matrices, contributing to the maintenance of feed safety and regulatory compliance within the European Union.
This high-performance liquid chromatography (HPLC) technique endorsed by the European Union Reference Laboratory (EURL) and the Community Reference Laboratory (CRL-FEED) is used for determining L-arginine in different feed-related matrices such as feed additives, premixtures, compound feed, and water. The EU method, complemented by the EN ISO 17180 method based on ion exchange chromatography (IEC-VIS/FLD), plays a crucial role in maintaining feed safety and ensuring consistent control and verification across member states, as it aligns with the regulatory requirements for feed nutrition and health-and-wellness.
